RESUMO
REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes and DNA methyltransferases together with their commercial availability, methylation sensitivity, crystal and sequence data. All completely sequenced genomes and select shotgun sequences are analyzed for RM system components. When PacBio sequence data is available, the recognition sequences of many DNA methyltransferases (MTases) can be determined. This has led to an explosive growth in the number of well-characterized MTases in REBASE. The contents of REBASE may be browsed from the web rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.
Assuntos
Metilação de DNA , Metilases de Modificação do DNA , Bases de Dados Factuais , Enzimas de Restrição do DNA/metabolismo , Metilases de Modificação do DNA/metabolismo , DNA/genética , Enzimas de Restrição-Modificação do DNA/genéticaRESUMO
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.
Assuntos
Metilases de Modificação do DNA/metabolismo , Enzimas de Restrição do DNA/metabolismo , Bases de Dados Genéticas , Metilases de Modificação do DNA/química , Metilases de Modificação do DNA/genética , Enzimas de Restrição do DNA/química , Enzimas de Restrição do DNA/genética , Genes , Genômica , Internet , Análise de Sequência de DNA , SoftwareRESUMO
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification (R-M). It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal and sequence data. Experimentally characterized homing endonucleases are also included. The fastest growing segment of REBASE contains the putative R-M systems found in the sequence databases. Comprehensive descriptions of the R-M content of all fully sequenced genomes are available including summary schematics. The contents of REBASE may be browsed from the web (http://rebase.neb.com) and selected compilations can be downloaded by ftp (ftp.neb.com). Additionally, monthly updates can be requested via email.
Assuntos
Biologia Computacional/métodos , Metilases de Modificação do DNA/genética , Enzimas de Restrição do DNA/genética , Bases de Dados Genéticas , Sítios de Ligação , Biologia Computacional/tendências , Bases de Dados de Proteínas , Genoma Arqueal , Genoma Bacteriano , Genômica , Armazenamento e Recuperação da Informação/métodos , Internet , Estrutura Terciária de Proteína , Software , Especificidade por SubstratoRESUMO
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal and sequence data. Experimentally characterized homing endonucleases are also included. All newly sequenced genomes are analyzed for the presence of putative restriction systems and these data are included within the REBASE. The contents or REBASE may be browsed from the web (http://rebase.neb.com/rebase/rebase.ftp.html) and selected compilations can be downloaded by ftp (ftp.neb.com). Additionally, monthly updates can be requested via email.
Assuntos
Metilases de Modificação do DNA/química , Enzimas de Restrição do DNA/química , Bases de Dados de Proteínas , Metilases de Modificação do DNA/genética , Metilases de Modificação do DNA/metabolismo , Enzimas de Restrição do DNA/genética , Enzimas de Restrição do DNA/metabolismo , Genoma Arqueal , Genoma Bacteriano , Genômica , Internet , Especificidade por SubstratoRESUMO
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Experimentally characterized homing endonucleases are also included. Additionally, REBASE contains complete and up-to-date information about the methylation sensitivity of restriction endonucleases. An extensive analysis is included of the restriction-modification systems that are predicted to be present in the sequenced bacterial and archaeal genomes from GenBank. The contents of REBASE are available by browsing from the web (http://rebase.neb.com/rebase/rebase.html) and through selected compilations by ftp (ftp.neb.com) and as monthly updates that can be requested via email.
Assuntos
Metilases de Modificação do DNA/química , Enzimas de Restrição do DNA/química , Bases de Dados de Proteínas , Metilases de Modificação do DNA/metabolismo , Enzimas de Restrição do DNA/metabolismo , Genoma Arqueal , Genoma Bacteriano , Especificidade por SubstratoRESUMO
REBASE contains comprehensive information about restriction enzymes, DNA methyltransferases and related proteins such as nicking enzymes, specificity subunits and control proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Homing endonucleases are also included. REBASE contains the most complete and up-to-date information about the methylation sensitivity of restriction endonucleases. In addition, there is extensive information about the known and putative restriction-modification (R-M) systems in more than 100 sequenced bacterial and archaeal genomes. The data is available on the web (http://rebase.neb.com/rebase/rebase.html), through ftp (ftp.neb.com) and as monthly updates via email.
